Before Season 1
When the concept of subjection came into existence, Pollen came into existence. Before the Miraculous jewels were created, Pollen and the other kwamis traveled all over the universe unable to interact with humans. Then, thousands of years ago, a mage created the magic jewels. Pollen started out in China and has most recently been dormant inside the Bee Miraculous inside Wang Fu's Miraculous jewelry box.
As of season 1, Pollen laid dormant in the Bee Miraculous.
In "Style Queen", Pollen was still dormant inside the Bee Miraculous and was intended to be given to Alya Césaire by Ladybug. But before she and her Miraculous could be activated, Ladybug was attacked by Style Queen, causing her to drop it. As the Miraculous Ladybugs did not return the Bee Miraculous to Ladybug, Pollen was found by Chloé Bourgeois and, once activated, she greeted her wielder.
In "Queen Wasp", Chloé, in an attempt to impress her mother, activated the Bee Miraculous in front of everyone and commanded Pollen to transform her into Queen Bee. After being exposed as an irresponsible hero and humiliated by her own mother, Queen Bee is akumatized, with the akuma possessing the Bee Miraculous while Pollen was still charging it. After the akuma was taken out by Cat Noir's Cataclysm, the Bee Miraculous was momentarily destroyed but then restored by Ladybug's Miraculous Ladybug with Pollen dormant inside. Chloé then returned the Bee Miraculous to Ladybug.
In "Malediktator", Pollen was delighted to meet Chloé again and helped her transform into Queen Bee. With Ladybug, a cat-like Cat Noir, and her Venom power the trio was able to stop the villain. After Malediktator’s defeat, Pollen went dormant inside the Bee Miraculous once again and was returned to Ladybug and then to Master Wang Fu.
In "Sandboy", Pollen was celebrating Nooroo's birthday inside the Miracle Box with the other kwamis and was excited when Tikki and Plagg arrived. She formally greeted Tikki and Plagg and marveled at the gift Tikki brought and played with the other kwamis. When the time came to contact Nooroo, Pollen got into position with the other kwamis but they ended up contacting his wielder instead. Upon hearing Hawk Moth's voice, she shuddered at the thought of being his slave and to prevent their location from being discovered by him, all the kwamis cut off the connection. However, she and the other kwamis realized that if they contacted Nooroo's wielder, then someone must have been akuamtized and that Tikki's and Plagg's wielders were in danger.
During "Catalyst (Heroes' Day - Part 1)", when Scarlet Moth unleashed an army of akumatized villains on the city, Pollen and her Miraculous were retrieved by Ladybug and given to Cat Noir, who passed it onto Chloé to transform into Queen Bee.
In "Kwamibuster", she was activated along with her kwami brothers and sisters to help Multimouse enact her plan of rescuing Tikki and Plagg. During the confrontation, Pollen was one of the few kwamis captured but was actually part of the plan, as she unveiled the another Multimouse and was set free with the help of Multicat. After Kwamibuster's defeat, Pollen and others cheered for Marinette as their plan was a success and was returned to Miracle Box.
In "Heart Hunter (The Battle of the Miraculous - Part 1)", upon meeting Chloe again, Pollen was shocked that she was siding with Hawk Moth and tried convince her not to do this, but silenced by her wielder and transformed her into Queen Bee.
In "Miracle Queen (The Battle of the Miraculous - Part 2)", after Chloe was deakumatized as the titular villain and upon having the Bee Miraculous taken off her, Pollen and Chloe detransformed. Then, she heard her former wielder say that she will no longer be a fan of Ladybug's and rant on about wanting to leave for New York as well as watching her running away. Afterwards, Pollen and her Miraculous were returned to the Miracle Box.